INTESTINAL PARASITOSIS AMONG UNDERNOURISHED CHILDREN OF AN URBAN SETTLEMENT IN WEST AFRICA: PATTERN AND TYPES    [Original Research]
JOMBO GTA (FMCPath)1,  DAMEN JG (MSc)2,  AMECHI I (FIMLS)3,  ETUKUDO NS (FIMLS)3,  DABIT O (RN)4
1.Department of Medical Microbiology and Parasitology, College of Health Sciences, Benue State University, PMB 102119 Makurdi, Nigeria.
2.Department of Medical Laboratory Science, Faculty of Medical Sciences, University of Jos, Nigeria.
3.Federal School of Medical Laboratory Science, Jos, Nigeria.
4.Department of Paediatrics, Jos University Teaching Hospital, Jos, Nigeria.

[emedpub – International Infectious Diseases :   Vol 1:1]                  [Date of Publication: 03.18.2011]
ISSN 2231-6019

March 18, 2011 at 7:56 PM

Abstract :

Background: Human intestinal parasites and malnutrition still constitute a major health challenge in several parts of sub-saharan Africa with the attendant clinical and social impact on the people. This study was set up to ascertain the level of intestinal parasitosis among undernourished children in Jos, Nigeria with a view of improving the quality of their medicare.

Patients and Methods: The study was hospital based. Children aged 6 to 70 months attending Paediatric clinic in Jos, Nigeria comprising 311 undernourished and 97 controls were consecutively recruited into the study. Relevant information on the patients and their respective caregivers such as age, sex, occupation, educational levels and wealth index were obtained with the aid of structured questionnaires while stool samples were collected, stored and processed using standard methods to identify stool parasites.

Results: The incidence of intestinal parasites among the undernourished children was 63.3% compared to 22.7% among the control (P<0.001). The most common parasites recovered were Entamoeba dispar (27.6%), Entamoeba coli (19.7%) , Cryptosporidium parvum (15.9%) and hookworm (14.5%). Ascaris lumbricoides (32.4%)  was the commonest parasite recovered from the control group. Besides malnutrition, low educational levels, poor sanitary conditions, and obviously economic factors were all contributory to the high parasite burden.

Conclusion: Health education on personal and environmental hygiene should be intensified and avenues to improve literacy levels pursued while mass chemotherapy with antihelminths be seriously considered in the interim period.

Introduction :

It is generally estimated that at least 2.5 billion of the estimated world’s 6.9 billion people are currently infected with intestinal parasites cutting across all continents and regions of the world [1-3]. These parasites of which Enterobius vermicularis, Giardia lamblia, Ancylostoma duodenale, Necator americanus and Entamoeba dispar are generally the commonest globally and account for over 70% of the intestinal parasitic load in the tropical and sub-tropical regions of the world [4-6]. In some communities in sub-saharan Africa, infection rates as high as 70% of the entire population has been documented, thus still making the disease of serious public health importance due to its direct and indirect association with the prognostic outcome of several diseases [7,8].

The first global initiative to fight intestinal parasites was constituted by John D Rockefeller, a philanthropist per excellence in New York city through Rockefeller foundation’s International Health Board (IHB) in 1909 who used the potentially poisonous oil of chenopodium to expel hookworm thought to be responsible for the backwardness of then southern USA [9]. The outcomes generally beneficial were often disastrous with fatal outcomes. The activities of IHB were eventually taken over by the world health organization (WHO) in 1945 [9,10].

Presently the control of intestinal parasites has been decentralised to governmental and non-governmental organizations, international organizations, community-based organizations, health institutions, philanthropists and private individuals all working towards common goal to rid planet earth of the present parasite burden through health education, provision of quality water, improvement in personal and environmental hygiene as well as qualitative and quantitative food security [11-13].

Malnutrition has been adjudged one of the commonest childhood ailments in Nigeria and several parts of sub-saharan Africa and directly or indirectly accounts for most of the paediatric outpatient presentations as well as admissions. It is also a fact that in most parts of sub-saharan Africa facilities for both mother and child are often in short supply and facing various challenges of quantity and quality. The impact of HIV/AIDS on children has also been largely attributed to their nutritional status which influences the course and rate of progression of the disease. This makes malnutrition an important co-morbidity to several diseases of both adult and children [14-16].

A clear knowledge of the types of parasites prevalent among undernourished children would be useful in the management plan of medicare comprising nutritional and metabolic needs as well as a correct treatment of their infections and infestations especially in settings where facilities for comprehensive laboratory diagnosis may be lacking or are in short supply [17-19]. It is in this regard that the present study was set up to assess the burden and types of intestinal parasites among undernourished children presenting at a University hospital in Nigeria, West Africa.

Patients And Methods :

Setting. The study was carried out in Jos, the capital city of Plateau state located in north central Nigeria between August and April 2009. Based on 2006 population census the city has an estimated population of 800,000 people and has a temperate-like climate with captivating hills surrounding it. Among several other large and small health institutions located in the city is the Jos University Teaching hospital (JUTH) which offers primary, secondary as well as tertiary health-care to the people. JUTH is also a referral centre for neighbouring states such as Nasarawa , Benue, Gombe , Taraba and Federal Capital Territory Abuja.

Procedures. The study was carried out at the Jos University Teaching hospital (JUTH) on undernourished children who were between the ages of six months and five years old attending the Paediatric outpatient clinic or General outpatient clinic. Their nutritional status were assessed using weight – for – age, weight for height and height for age measurements. The measurements were expressed on standard deviations above or below the median value for corresponding sex and age of WHO reference population. Participation in the study was purely voluntary, verbal consent for participation was obtained from their mothers or accompanying adults. A questionnaire was administered on each participant and relevant information as age and sex of subjects, occupation of mothers or care givers and educational levels, methods for prevention of intestinal parasites, sources of drinking water and methods of sewage disposal were obtained [20].

Specimen Collection and Processing. A clean wide mouth, transparent, dry and disinfectant-free universal container was given to accompanying relatives of undernourished children and the process of collecting stool specimen was explained to them. Each of the stool specimens was examined macroscopically and microscopically using saline preparation and Lugol’s Iodine staining procedure for wet mount. Formal ether concentration technique was used as the concentration procedure. Samples were examined microscopically using X5 and X10 objective lenses and rarely X40. Parasite density in terms of their number per medium power (X10) field of uncentrifuged stool specimen and number of species per stool specimen were also noted. Another group of 97 children age and sex matched were consecutively selected as control group whose stool specimens were also collected and analysed microscopically [21].

Analysis. Data obtained was analysed using Pearson Chi square, Mantel-Haenszel Chi square, Regression and Analysis of variance (ANOVA) where applicable, p-values were considered significant at 95% confidence interval.

Ethical Considerations Ethical approval for the study was obtained from the Institutional Health Research ethical committee of the Jos University Teaching Hospital (JUTH). Verbal or written consents were obtained from informants of subjects for the study before voluntary enrollment.

Results :

A total of 311 undernourished children were studied comprising 150 males (48.2%) and 161 females (51.8%). The age range of the subjects was 6 to 70 months with mean age of 26 months, median age of 40 months and modal age of 29 months old. The incidence of intestinal parasites among the undernourished children was 197/311 (63.3%) while that among the nourished children was 22/97 (22.7%) {X2 (Mantel-Haenszel)= 17.44, OR= 0.60, RR= 0.71, p< 0.0001}.

Analysis of age and gender distribution of intestinal parasites among undernourished children showed that the age range with the lowest rate of infection was those aged less than 10 months old {1 (11.1%) male and 2 (22.2%) females} with no significant age association (p> 0.05). Similarly, the rate of infection among males was 109/150 (72.6%) and that among females was 88/161 (54.7%) with no significant gender difference (p> 0.05) (Table 1).

The most common parasites recovered from stool specimens of undernourished children were Entamoeba dispar 27.6%, Entamoeba coli 19.7%, Cryptosporidium parvum 15.9%  and hookworm 14.5%. Others were Giardia lamblia 10.5%, Ascaris lumbricoides 9.2% and Trichuris trichiura 2.6%. Ascaris lumbricoides 32.4% was the commonest parasite recovered from the control group (Table 2).

A review of the species of parasites per stool sample among the infected undernourished subjects showed that 74.1% were infected with one parasite species, 16.3% with two parasite species, and 9.6% with three or more parasites species. Multiple intestinal parasite infections were not common findings among the nourished children {X2 (Mantel-Haenszel)= 19.34, OR= 0.71, RR= 0.92, p< 0.0001} (Figure 1).

Analysis of the socio-demographic factors associated with intestinal parasites among undernourished children showed that knowledge of methods of prevention, methods of sewage disposal, educational levels of mothers or care givers and wealth index were important contributory factors towards infection (p< 0.05). Also occupation of mothers or care givers such as petty trading, artisans and farming were associated with significantly higher rates of infection among their children compared to others like civil servants and health workers (p< 0.05) (Table 3).

Discussion :

The incidence of intestinal parasites infestation among undernourished children in Jos was 63.3% while that among the control was 22.7%  (p< 0.05). Methods of sewage disposal such as trenches, open air defaecation, low educational levels of care givers, inadequate knowledge about prevention of intestinal parasites, and economic factors were found to be important contributory factors to the high rate of infection (p< 0.05).  Sources of drinking water being either tap, bore hole, shallow well or stream did not significantly influence the rate of infections (p>0.05). The most common parasites recovered from stools of malnourished children were Entamoeba dispar 27.6%, Entamoeba coli 19.7%, Hookworm 14.5% while Trichuris trichiura 2.6% was the least common. These findings have shown that intestinal parasitic infections are still of serious public health importance in the community.

The findings from the present study compare well with that from related studies in Onitsha, Ondo and Lagos in Nigeria where infection rates of 44.5%, 40.9% and 70.6% respectively, were documented [22-24]. Also similar high rates of 57.0% and 95.0% respectively were reported from separate studies in Guinea and South African rural dwellers [25,26].  The combined effect of water and environmental sanitation on high intestinal parasitic load among Africa’s both urban and rural dwelling children has elaborately been documented. The fact that quality and sources of water for domestic use in the present study had little impact on intestinal parasite load shows that more needs to be done in the area of public enlightenment on the benefits derivable from quality water utilization. Also other factors such as sewage disposal facilities and occupational inclinations of care givers could have masked the potential benefits of quality water supply [27].

With the high rate of intestinal parasites among undernourished children in the present study, dietitians and clinicians  should emphasise important components of personal hygiene such as frequent hand washing, washing of all fruits and vegetables with clean water before eating raw as well as boiling and filtering of water before drinking. This would reduce the general parasite load among children, including the undernourished one who bear a greater brunt of the disease [28-30].

The findings from the present study are partly different from that of: Adamu in Ethiopia [31] where parasites such Blastomycis hominis, Hymenolepsis nana and Encephalitozoon intestinalis were recovered from children with diarrhoea [32]; Ascaris lumbricoides was the most frequently recovered parasite in stool specimens of under-fives in Malawi [33] and in Brazil [34]; and  Crytosporidium parvum was recovered from over 50% of animal handlers in Iraq [35]. The high rate of C. parvum and Isospora belli recorded in Ghana [36], and Ethiopia [37,38] is understandably from patients with HIV/AIDS. Besides the geographical species variations and morbid and pre-morbid conditions associated with intestinal parasitosis, the disease burden is generally high across entire sub-saharan Africa [39-41].

The present study is limited by the fact that the HIV-serostatus of the subjects was not ascertained so as to document its contributory role. However, studies have shown that intestinal parasite load is not significantly higher in HIV-seroconverted carriers whose immune status is still intact compared to the HIV-seronegative subjects [42-44].

Recommendations :

Due to the porous knowledge about prevention of intestinal parasites public enlightenment should be intensified with emphasis on regular hand washing, boiling of drinking water as well as proper ways of sewage disposal. Government and other voluntary organizations should build public toilets in the city’s strategic locations with proximity gauged to serve the sanitary needs of the people. Provision should be made for their regular maintenance and the public encouraged to use them at minimal or no cost. Strategies for garbage and refuse dump clearance should be mapped out and efforts intensified to secure a clean and habitable environment.

Education should ensure that school curricula right from elementary classes upwards to probably the general studies courses at university entry should emphasise strongly on environmental health, hygiene and sanitation. This would internalise the culture of personal and environmental sanitation among children as they grow to adulthood to become care givers and parents.

Facilities for adult formal and informal education should be provided and adults carefully mobilized to enroll in order to raise their literacy levels. Curricula should as well be designed to cover the key aspects of environmental health, hygiene and sanitation.

Mass treatment should be carried out non-selectively on all school children using deworming medications such as albendazole and mebendazole which are relatively safe. Pre-school children could be reached through local community-based or religious organizations and other social gatherings so as to prevent a re-introduction of new infections in schools from new entrants.

Mass treatment with deworming medications is also strongly advocated for both adults and children in communities so as to break the chain of infection and re-infection. Government should assist in the distribution of anti-helminths through recruitment of community medicine distributors (CMDs) and other volunteers who would carry out house to house medications on regular intervals.

The overall impact of a successful implementation of these recommendations with appropriate regular and periodic surveillance will lead to a drastic reduction of worm load among the paediatric group in the community.

References :

1. Morales-Espinoza EM, Sanchez-Perez HJ, Garcia-Gil MM, Vorgas-Morales G, Mendez-Sanchez JD, Perez-Ramirez M. Intestinal parasites in children in highly deprived areas in border region Chiapas, Mexico. Salud Publica Mex 2003; 45(5): e. doi: 10. 1590/S0036-36342003000500008.

2. Kucik CJ, Martin GL, Sorter BV. Common intestinal parasites. Am Fam Physician. 2004; 69: 1161-1168.

3. Okodua M, Adeyeba A, Oluwaseyi T, Youtcho O, Herbert M. Age and sex distribution of intestinal parasitic infection among HIV-infected subjects in Abeokuta, Nigeria. Online J Health Allied Sci 2004; 2(4): e.

4. Geltman PL, Cochran J, Hedgecock C. Intestinal parasites among African refugees re-settled in Massachusetts and the impact of an overseas pre-departure treatment programme. Am J Trop Med Hyg 2003; 69(6): 657-662.

5. Hunter G, Bagshawe AF, Baboo KS, Luke R, Praciv P. Intestinal parasites in Zambian patients with AIDS. Trans-R-Soc Trop  Med Hyg 1992; 86(5): 543-545.

6. Agbolade OM, Akinboye DO, Awolaja A. Intestinal helminthiasis and urinary schistosomoasis in some villages of Ijebu-north, Ogun state Nigeria. Afr J Biotechnol 2004; 3(3): 206-209.

7. Ameh IG, Onah JA, Aman RM. Intestinal parasites: positive cases and low haematocrit among pregnant women at the ante-natal clinic in Vom, Nigeria. Niger J Parasitol 2004; 25: 33-37.

8. Adams VJ, Markua MB, Adams JFA, Jordaan E, Curtia B, Dhansay MA, et al. Paradoxical helminthiasis and giardiasis in Cape-town, South-Africa. Epidemiology and control. Afr Health Sci 2005; 5(2): 131-136.

9. Bond AK. Death after wormseed. Maryland Med J 1997; 37: 289-290.

10. Roth DA. Some dangers of the chenopodium treatment. South Med J 1918; 11(11): 733-734.

11. Mbanugo JI, Onyebuchi CI. Prevalence of intestinal parasites in Ezinifite community, Aguata local government area of Anambra state. Niger  J Parasitol 2002; 23(1): 27-34.

12. Esray SA, Potash JB, Roberts L, Shiff C. Effects of improved water supply and sanitation on ascariasis, diarrhoea, dracunculiasis, hookworm infection, schistosomiasis and trachoma. Bull World Health Organ 1991; 69(5): 609-621.

13. Itah AY, Opara KN, Atting IA, Udoidung NI. Prevalence of enteropathogens and their association with diarrhoea among food vendors in Uyo, Nigeria. Mary Slessor J Med 2005; 5(1): 11-21.

14. Udo SM, Mbato CI, Eja ME, Ekanem EE. Association of multiple intestinal parasites and some specific parasites with human immunodeficiency virus sero-positive status in Calabar. Global J Med Sci 2003; 2(1): 7-11.

15. Addy PAK, Andepin G, Frimpong EN. Prevalence of pathogenic Escherichia coli and parasites in infants with diarrhoea in Kumasi, Ghana. East Afr Med J  2004; 81(7): 353-357.

16. Awole M, Gebre-Selassie S, Kassa T, Kibru, G. Prevalence of intestinal parasites in HIV-infected adult patients in southwestern Ethiopia. Ethiopia J  Health Dev 2003; 17(1): 71-78.

17. Hotez PJ. Neglected infections of poverty among the indigenous people of the Arctic. PLoS Negl Trop  Dis 2010; 4(1): e606. doi: 10. 1371/journal.pntd.0000606.

18. Ndao M. Diagnosis of parasitic diseases: old and new approaches. Interdiscip Perspect Infect  Dis 2009; 2009: e278246. doi: 10. 1155/2009/2009/278246.

19. Jombo GTA, Damen JG, Safiyanu H, Odey F, Mbaawuaga, E.M. Human intestinal parasitism, potable water availability and methods of sewage disposal among nomadic Fulanis in Kuraje rural settlement of Zamfara state. Asian Pacific J Trop  Med 2010; 3(6): 491-493.

20. Jombo, G.T.A., Egah, D.Z., Akosu, J.T., and Mbaawuaga, E.M. Human intestinal parasitism in a rural settlement of northern Nigeria, a survey. Niger Med Pract 2007; 51(1/2): 11-14.

21. Jombo GTA, Egah DZ, Akosu TJ. Intestinal parasitism, portable water availability and methods of sewage disposal in 3 communities in Benue state: a survey. Ann  Afri Med 2007; 6(1): 17-21.

22. Ekejindu IM, Ochuba GC. Cryptosporidium infection among children in Onitsha urban area in south-eastern Nigeria. Trop  J  Med Res 2004; 8(1): 17-20.

23. Dada EO, Erinle BA. Study of human gastrointestinal parasites among primary school children in Ibule Soro-community of Ifedore local government area of Ondo state, Nigeria. J Med Lab Sci  2004; 13(1): 62-65.

24. Salako AA. Effects of potable water availability on intestinal parasitism among rural school children with sewage disposal facilities in the Majidun and Owutu sub-urban community of Lagos state. Niger  Med Practi 2001; 39(3/4): 30-35.

25. Glickman LT, Camara AO, Glickman NW, McCabe GP. Nematode intestinal parasites of children in rural Guinea, Africa: prevalence and relationship to geophagia. Int  J Epidemiol 1999; 28: 169-174.

26. Soave R. Editorial response: Waterborne cryptosporidiosis-setting the stage for control of an emerging pathogen. Clin  Inf Dis 1995; 21: 62-61.

27. World Health Organization. Prevention and control of intestinal parasitic infections, Geneva: 1987 WHO Technical report series, N249.

28. Adeyeba OA, Akinbo JA. Profile of potentially pathogenic intestinal parasites and bacterial agents in solid wastes in Ibadan municipality. Afr J Clin and Experimental Microbiol 2003; 4(1): 31-43.

29. Anosike JC, Ebiziem N, Ajero CM, Asor JE, Adeiyongo CM, Bolaji OS. et al.  Prevalence and public health significance of helminths ova in deposited dog faeces in Owerri, Nigeria. Animal Production Research Advances 2006; 2(1): 34-38.

30. Talabi AO, Oyekunde  MA, Onasanya AS, Tijani LA, Sosanya OS, Ettu RO. (2004).  Comparison of the efficacies of diazinon + albendazole, ivomec and ivojec on the control of gastro-intestinal nematodes and ectoparasites of pigs. Afr J  Livestock Extension 2004; 3: 55-58.

31. Adamu H, Endeshaw T, Teka T., Kiffe A, Petros B. The prevalence of intestinal parasites in paediatric diarrhoeal and non-diarrhoeal patients in Addis Ababa hospitals, with special emphasis on opportunistic parasitic infections and with insight into the demographic and socio-economic factors. Ethiop  J Health Devel 2000; 20(2): 39-46.

32. Ogbonna C, Okolo SN, Okonji MC. Intestinal worms and nutritional status of under fives in Jos, Nigeria: any relationship? Nigerian J Clin Pract 2004; 7(2): 79-81.

33. Phiri KS. The prevalence, intensity and ecological determinants of helminth infection among children in an urban and rural community in southern Malawi. Malawi Med J 2001; 13(3): 22-26.

34. Mascarini-Serra LM, Telles CA, Prado MS, Mattos SA, Strina A, Alcantara-Neves NM et al. Reduction in the prevalence and incidence of geohelminth infections following a city-wide sanitation programme in a Brazilian urban centre. PLoS Negl Trop Dis 2010; 4(2): e588.

35. Mahdi NK, Ali NH. Cryptosporidiosis among animal handlers and their livestock in Basrah, Iraq. East Afr  Med J 2002; 79(10): 551-554.

36. Adjei A, Adiku TK, Rodrigues O, Renner L, Sifah E, Mensah JD, et al. Cryptosporidium oocysts in Ghanian AIDS patients with diarrhoea. East Afr Med J 2003; 80(7): 369-372.

37. Mekonnen E, Abate E, Beyene G, Kassu  A. Disseminated strongyloidiasis among HIV/AIDS patients in Jimma. J Ethiopian Med Pract 2001; 3(2): 81-84.

38. Abate E, Mekonnen E, Awol M, Kassu A. Cryptosporidiosis and Isosporiasis among HIV/AIDS patients in Jimma, southwest Ethiopia. J Ethiopia Med Pract 2001; 2(2): 64-69.

39. Ouattara M, N’Guessan NA, Yapi A, N’Goran EK. Prevalence and spatial distribution of Entamoeba histolytica/dispar and Giardia lamblia among schoolchildren in Agboville area (Cote d’Ivoire). PLoS Negl Trop Dis 2010; 4(1): e574. doi: 10.1371/journal.pntd.0000574.

40. Mehta S, Giovannucci E, Mugusi FM, Spiegeman D, Aboud S, Hertzmark E, et al. Vitamin status of HIV-infected women and its association with HIV disease progression, anaemia and mortality. PLoS One. 2010; 5(1): e8770. doi: 10. 1371/journal.pone.0008770.

41. Fincham JE, Markus MB, Brombacher F. Vaccination against helminths: influence on HIV/AIDS and TB. Trends Parasitol 2002; 18: 385-386.

42. Elias D, Wolday D, Akuffo H, Petros B, Bronner U, Britton S. Effects of deworming on human T-cell responses to mycobacterial antigens in helminth-exposed individuals before and after Bacille Calmette-Guerin (BCG) vaccination. Clin Exper. Immunol 2001; 123: 219-225.

43. MacDonald AS, Araujo MI, Pearce EJ. Immunology of parasitic helminth infections. Infect Immun 2002; 70: 427-433.

44. Tristo SR, Ribeiro-Rodrigues R, Johnson LT, Pereira FEL, Dietze, R. Intestinal nematodes and pulmonary tuberculosis. Rev Soc Brasil Med Trop 2002; 35: 533-535.

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